PP 16. Improvement Of Colorectal Anastomotic Healing By Hyperbaric Oxygen Therapy

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G. Boersema, Z. Wu, S. Vennix, L. Kroese, G. Kleinrensink, H. Jeekel, J. Lange

18:10 - 18:16h at Margrit Room

Categories: Gastrointestinal Surgery, Poster Session , Robotic and Minimal Invasive Surgery

Session: Poster (P2) - Gastrointestinal Surgery / Minimal Invasive

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Background
Hyperbaric oxygen treatment (HBOT) has been found to improve the healing of tissues which are poor in oxygen. This study aims to investigate the influence of HBOT on the healing in ischemic colorectal anastomosis.

Material and Methods
We operated 4 groups with 10 Wistar rats each. Group A and B received HBOT for 10 days (7 days before surgery, 3 days after), short term (3 days) and long term (7 days) follow up, respectively. Group C and D did not receive HBOT and had also a follow up period of 3 and 7 days. In all groups a partial colectomy with ischemic cutting edges was performed and proximal and distal ends of the colon were anastomosed inverted.

Result
5 Rats from HBOT groups and 5 rats from control groups died during follow up. Kidney function (creatinin) of the HBOT groups was significantly better compared to control groups on day 7 (p<0.05). There was no anastomotic leakage in the HBOT groups, comparing with 44.1% and 16.7% detachment in group C and D, respectively. Group A had a significant higher bursting pressure (130.9 ± 17.0 mmHg) than group C (87.8 ± 43.7 mmHg; p = 0.046). The adhesion severity was significant higher in the control groups than the HBOT groups on day 3 and day 7 (p < 0.005).

Conclusion
Application of hyperbaric oxygen therapy may facilitate colorectal anastomotic healing and improve the kidney function in patients with ischemic anastomoses.