Background:
Vascular operations on lower extremities cause ischemic-reperfusion (IR) injury and rhabdomyolysis of skeletal muscle, which could induce remote kidney injury. NIM-811 has mitochondria specific effects. Our aim was to reduce damages in skeletal muscle and in the kidney after IR of the lower limb with NIM-811.

Material and Methods:
Wistar rats underwent 180 minutes bilateral lower limb ischemia and 240 minutes reperfusion. Animals were divided into four groups called Sham (vehicle; n= 10pcs), NIM-sham (NIM- 811+vehicle; n=10pcs), IR (vehicle+IR; n=10pcs), and NIM-IR (NIM-811+vehicle+IR; n=10pcs). Serum, urine and histological samples were taken. NADH-tetrazolium staining, muscle Wet/Dry (W/D) ratio calculations, and laser Doppler-flowmetry (LDF) were performed. Renal peroxynitrite concentration, serum TNF-α and IL-6 levels were measured.

Result:
Lesser extent of histopathological alterations was presented in the NIM-IR group compared to the IR group. The serum necroenzyme levels were significantly lower in the NIM-IR group than in the IR group (LDH:p=0.01; CK:p=0.04). Muscle mitochondrial viability proved significantly higher (p<0.001) and renal function parameters were significantly better (creatinin:p=0.002; FENa:p=0.01) in the NIM-IR group compared to the IR group. Level of serum TNF-α was significantly lower (p=0.04), as well as W/D ratio and peroxynitrite concentration were significantly lower (W/D:p=0.04; peroxynitrite concentration:p=0.003) in the NIM-IR group compared to the IR group.

Conclusion:
NIM-811 could have the potential of reducing rhabdomyolysis and the impairment of the kidney after lower limb IR injury.