PP 63. HMGB1 Promotes the Expression of Autophagy Signal Beclin1 in Acute Pancreat

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C. Yu, X. Yu

18:28 - 18:34h at Lanchid Room

Categories: Hepatobiliary and Pancreatic Surgery, Poster Session

Session: Poster (P5) - Hepatobiliary & Pancreatic Surgery

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Background
To study the significance of HMGB1 expression in acute pancreatitis with excessive activation of autophagy key molecule Beclin1, explore the mechanism of the occurrence and development of acute pancreatitis, and thus provide a new theoretical basis for its clinical treatment.

Material and Methods
SD rats were randomly divided into sham-operated group and the experimental group which were injected with 5% sodium taurocholate into the bili-pancreatic duct retrogradely to produce ANP models, for SO group with equal dose of saline.. Change of the autophagosome in pancreatic acinar cells in acute pancreatitis was observed under electron microscopy. Immunohistochemical method was used to detect the distribution of HMGB1 and Beclin1 in pancreatic tissue. The expression of HMGB1 and Beclin1 in the pancreatic tissue was detected by Western blot and Q-PCR methods.

Result
Acute pancreatitis modeling was successful. HMGB1 in the ANP group had no significant change at 0h, 3h and 6h (P>0.05), significantly increased at 12h, and continued to rise at 24h with statistical significance (P<0.05). For the Beclin1 expression in the pancreatic tissue of ANP group, with the time course and aggravation of inflammation, it continued to increase significantly (P<0.05)

Conclusion
(1) Different from early inflammatory factors, HMGB1 expression increases significantly at late stages in the development of acute pancreatitis; (2) HMGB1 may play a role in the maintenance and promotion of the expression of autophagy signaling molecule Beclin1 in acute pancreatitis, thus contributing to the progression of acute pancreatitis.