Background
Infection affecting the skin and subcutaneous tissue is very common among people of all ages. The aim of this study is to evaluate microorganisms involved in wound infections in intensive care units and surgery clinics and their antibiotic resistance pattern.

Material and Methods
This study was conducted in surgical departments (general surgery, orthopedic, urology, plastic surgery, heart and vascular surgery) and intensive care units of our hospital. Wound infections were recorded in 443 patients. All samples were subcultured to blood agar and eosin methylene blue (EMB) agar. Microorganisms were identified by conventional methods and automated systems (Vitek 2, bioMerieux) and antibiotic susceptibility were evaluated.

Result
Pathogen microorganisms were cultivated from 128 (%28.9) patient samples. Gram-positive microorganisms constituted 45.3% (58 patients) of the pathogens responsible for wound infection and gram negative microorganisms constituted %53.1 (68 patients) of the pathogens. The rate of positive samples were highest in intensive care unit patients (%28.1) and general surgery patients (%23.4). The most commonly isolated microorganisms were Coagulase Negative Staphylococcus (CNS) (.75), Staphylococcus aureus (.2) and Escherichia coli (.2). Methicillin resistance in Staphylococcus aureus and CNS was .2 and %61.9, respectively. Resistance to penicillin, amoxicilline/clavulanic acid and tetracycline was high for gram positive bacteria and resistance to trimethoprim/sulfamethoxazole, cefazolin and ceftriaxone was high for gram negative bacteria. Acinetobacter spp. isolates had highest rate of resistence to antimicrobials.

Conclusion
This study indicates that antibiotic susceptibility tests are crucial in the treatment of patients with wound infection.