Ischemia-reperfusion injury (IRI) is inevitable during transplantation leading to oxidative stress. We previously reported that preoperative dietary restriction (DR), 3-day fasting and protein-free diets protect against IRI while a fat-free or carbohydrate-free (CHO-free) diet do not. To understand underlying mechanisms, we performed microarray analysis and compared gene expressions of (non)-protective diets in search for involved pathways.

Male C57BL/6 mice were randomized to preoperative normal food or:2 weeks 30%DR or a 3- day fasting, protein-free, CHO-free or fat-free diet. Gene expressions of kidneys after each diet were analysed by Affymetrix/Ingenuity. Cut-off for significance was set on fold