Background
The use of mouse models in the experimental research field is of enormous importance for the study of hepatic physiology and pathophysiological disturbances.

Materials
6-0 silk, 6-0 Prolene, 7-0 Prolene, 10-0 Prolene. Micro-forceps, needle holder, clamp and scissors.

Methods
2.1 Neck part
Dissect the jugular vein and the carotid artery and measure the blood flow artery using flow probe. Insert the millar catheter into the carotid artery for measuring the mean arterial pressure. Insert the fluid-filled catheter for measuring the central venous pressure.
2.2 Abdominal part
Measure the flow rate of common hepatic artery and portal vein using flow probe. Insert Millar catheter into a chosen mesenteric vein which has few branches and advance to the portal vein to obtain the PVP.

Result
Portal blood flow in normal mice ranged between 1.6 to 2.3ml/min; Flow in the hepatic artery ranged from 0.10 to 0.35 ml/min. Portal pressure was in the range from 4.4 to 11.2 cmH2O. Portal pressure before and immediately after 70%PH was 6.87±2.39 and 11.41±2.94 cmH2O. Clamping of the right lobe(20%) resulted in an increase of about 17%. Further clamping of the median and left lateral(90%) lobe caused an increase of at least 2-3 folds compared to the starting portal pressure. Portal pressure returned gradually to the starting point after releasing the clamp.

Conclusion
This full intraoperative monitoring procedure is needed to comprehensively understand hepatic physiological processes