PP 121. Protective Effect of Intestinal Ischemic Postconditioning

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K. Nedvig, J. Gábor, R. Erzsébet, S. Györgyi, C. Domokos, S. Jozsef, G. Wéber, A Ferencz

16:30 - 16:36h at Margrit Room

Categories: Organ and Cell Transplantation, Poster Session

Session: Poster II (P4) - Varia

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Background
Ischemia/reperfusion injury is a grave condition resulting from intestinal transplantation. The aims of our work was to follow up the oxidative stress parameters and tissue structural changes during intestinal autotransplantation testing the protective effect of ischemic postconditioning (IPO) especially to investigate the activation of transcriptional factor Nuclear Factor-kappaB (NF-kB).

Material and Methods
Total orthotopic autotransplantation was performed in Wistar rats (n=30). Grafts were stored in coldUniversity of Wisconsin solution for 1, 3 and 6 hours. Reperfusion took 3 hours. Prior to reperfusion IPO was performed by 3 cycles of 30 second ischemia and 30 second reperfusion. Tissue damage  was evaluated by Park's and software Scion Image on HE-stained sections. To monitor oxidative stress tissue malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) were determined. Cytoplasmic and nuclear NF-kB level were detected by ELISA method.

Result
IPO significantly decreased the intestinal wall injury (p<0.05). Meanwhile, the reperfusion-ended MDA's value was lower and the capacity and activity of endogenous antioxidant protective systems (GSH: 789±8.2 vs. 834±5.1 umol/g; SOD: 110±9 vs. 126±4.4 IU/g; p<0.05) remained higher in IPO groups. Total and activated form of NF-kB significantly elevated 30 minutes following IPO. 1 hour after IPO its activation decreased to the control level, however, after 2 hours gradual activation of NF-kB was detected again.

Conclusion
Three cycles of IPO prior to reperfusion could moderate intestinal oxidative and structural injury inducing intracellular signalling pathways on autotransplanted model.