PP 125. Experimental Small Bowel Allotransplantation in Rats: Review uf Surgical Techniques
J. Arvin, A Nickkholgh, S. Weih, G. Frongia, A Mehrabi
16:54 - 17:00h
at Lanchid Room
Categories: Organ and Cell Transplantation, Poster Session
Session: Poster II (P4) - Varia
Background
Small bowel transplantation has become the definitive surgical therapy for irreversible intestinal failure.SBTx still faces technical,therapeutic challenges that limit its success and clinical applications. Experimental studies are therefore still needed to address various challenges in the field of SBTx and Rat is the most ideal model for the purpose.
Material and Methods
An analysis of all Medline-published papers regarding SBTx in the rat model was performe dregarding the characteristics of animals, methods of animal care, techniques of procurement, recipient procedures, arterial and venous reconstructions, intestinal exteriorization, and posttransplant care.
Result
Generally, young male Sprague-Dawley (ideal for microsurgical procedures)or Lewis (ideal for physiological and immunological studies) rats were used. Irrigation of the bowel lumen with and systemic heparinization is usually performed. Flushing of the graft has been performed through superior mesenteric artery, aorta, or ileocolic artery. Iced lactated Ringer's solution, normal saline,
University of Wisconsin, or Histidine-Tryptophan-Ketoglutarate has been used as perfusion solution. Grafted segment was frequently selected from ligament of Treitz to ileocecal valve. SMA or aortic Carrel patch have been frequently used for the arterial revascularization into recipient's abdominal aorta (E/S), renal artery after a recipient nephrectomy (E/E), or even recipient's common carotid artery. Superior mesenteric vein or portal vein has been used for venous drainage into the recipient's IVC, portal vein, or renal vein.
Conclusion
The researchers may be better able to select and standardize the required techniques depending on their aims and objectives.
