OP 126. New Vessel-Lumen Filling Technique, for the Study of Neo- Microvascularisation of Autotransplanted “Spleen Chips”

---

M. Kiss, A. Nemeskeri, Z. Kürti, E. Gáti, B. Dorogi, I. Dudás, I. Furka, K. Nemeth, K. Pető, E. Ványolos, A Furka, I. Mikó

Chair(s): Attila Balogh, Thomas Hubert & Krisztián Szigeti

16:00 - 16:10h at Erszebet Room (A)

Categories: Oral Session, Abdominal Wall and Hernia

Session: Oral Session XIV - Surgical Anatomy

---

Background
After splenectomy the protection of the body against infections can be seriously damaged. To maintain the function of the spleen, autotransplantation was developed at the Debrecen University (Furka's method). For the investigation of the morpho-functional capacity of the splenic autotransplants, a new method was needed. Aims: To develop a new vessel-lumen filling technique on Beagle dogs for the study of the neomicrovascularisation of splenic autotransplants placed into the omentum majus.

Material and Methods
In one Beagle dog, after spleen chips autotransplantation into the omentum majus, on the 72nd postoperative months, the abdominal organs were filled with special resin mixture. CT pictures were taken, some autotransplants were excised for biopsy. The arterial and venous systems of the abdominal organ complex removed from a splenectomized Beagle dog were filled with colour coded resin after 60 postoperative month and the soft tissues were removed with KOH solution.

Result
Our technique allows the detailed investigation of the rich neo-microvascular network of the survived, autotransplanted spleen chips. On the CT scan, the vessel connection between the bearing tissue and the autotransplanted splenic tissue could be traced. Data on the 3D vascular morphology of the post-splenectomy liver were obtained.

Conclusion
The new vessel-lumen filling technique was suitable for study of the neo-microvascularisation of the adhered spleen autotransplants. This method combined with High-Resolution-CT is suitable to carry out a study with a higher number of cases. The data may contribute to the formation of the postexamination protocol of the future autotransplanted patients after posttraumatic splenectomy (OTKA-K-49331,OTKA-K-105618,HU).