WB 05. Intestinal Cold Storage In PACAP-38 Containing Preservation Solution
N. Klára, J. Gábor, S. Györgyi, C. Domokos, S. Jozsef, G. Wéber
Chair(s): Mustafa Cikirikcioglu, Frank Dor, Attila Szijarto, David J. Hackam, Cliff Shearman, Modise Koto, Yuzo Yamamoto
10:10 - 10:24h
at Erszebet Room (A)
Categories: Walter Brendel Award, Organ and Cell Transplantation
Session: Walter Brendel Award
Background
Small-bowel is one of the most sensitive organ to ischemia-reperfusion injury, which is a significant problem during transplantation. Pituitary adenylate cyclase-activating polypeptide (PACAP) has cytoprotective effect in ischemic injuries of various tissues. The aim of our study was to measure oxidative stress markers, histological damages and changes of PACAP-38 immunoreactivities and cytokine levels in intestinal grafts stored PACAP-38 containing preservation solution.
Material and Methods
Intestinal autotransplantation was performed on male Wistar rats (n=35). Grafts were stored in University of Wisconsin (UW) solution at 4°C for 1 hour (GI), for 3 hours (GII), and for 6 hours (GIII); and in PACAP-38 containing UW solution for 1 hour (GIV), for 3 hours (GV), and for 6 hours (GVI). After preservation reperfusion lasted 3 hours in each group. Tissue oxidative stress parameters (malondialdehide, reduced glutatione, superoxide dismutase), intestinal PACAP-38 immunoreactivities, and cytokine array were measured.
Result
Tissue oxidative injury and histological destruction were significantly lower in GIV-GIV compared to GI-GIII. Levels of PACAP-38 immunoreactivity decreased in GI-GII. This decrease was significant following 6 hours cold storage (p<0.05). Values remained significantly higher in grafts stored in PACAP-38 containing UW. Cytokine array revealed that expression of sICAM-1, Lselectin, and the tissue inhibitor of metalloproteinase-1 were increased in GIII, and strong reduction of their activation were observated in GVI.
Conclusion
Present study showed that PACAP-38 adding to the conventional UW preservation solution decreased intestinal oxidative injury and structural damages, and mitigated tissue cytokine expression.
